Role of extracellular mitochondria in adverse transfusion reactions

Role of extracellular mitochondria in adverse transfusion reactions


Platelets   are playing   critical  roles  in  hemostasis,   and  transfusion   of  platelets   is  a  life-saving therapeutical procedure for patients undergoing a major surgery, with massive bleedings, for subjects withabnormal platelets (not fully functional) and diseases such as cancers or thrombocytopenia.For platelet transfusion, it is necessary to prepare, store and maintain platelet concentrates. Platelets concentrates have a short shelf life and must be conserved at ambient temperature- ideal for bacterial growth.Bacterial contamination of platelet concentrates represents the major cause of morbidity and mortality in transfusion. However, the bacterial agent causing these effects remains to identify, and the plateletsthemselves might secrete inflammatory mediators promoting adverse reactions.

Recently, we found that platelets release their mitochondria in the extracellular milieu during storage in platelet concentrates serving for transfusion (findings protected by a patent held by Université Laval).Mitochondria   are thought   to  originate   from   the  endosymbiosis   of  proteobacterium (Alphaproteobacterium  Rickettsia prowazekii) during the development of the eukaryotic cells. We thus hypothesized that mitochondria  might  be  the  causal  agent  behind  the  transfusion  inflammatory reactions.  In this study,  we  aim  to  verify  the  presence  of  mitochondria  and  mitochondrial  derived molecules in thetransfused platelet concentrates in a prospective cohort study in order to link these prospective biomarkers with adverse effects. We will collaborate with the haematologists in charge of transfusion and will use state-of-the-art  methods  of quantification  of soluble mitochondria  in platelet storage bags. Our goal is to determine whether our simple tests can serve to assess platelet quality to limit transfusion adverse reactions.

 

Investigators


Jacques Corbeil

CHUdeQc/Université Laval

Éric Boilard

CHUdeQc/Université Laval

 

Funding


ULaval FMED                               logo_elul